Protein engineering and crystallography.
نویسندگان
چکیده
منابع مشابه
Protein engineering as a tool for crystallography.
The generation of large quantities of protein by overexpression technology has enabled structural studies of many important molecules that are found in only minute quantities in the cell. An increasing number of structures of proteins overexpressed in non-native systems have been solved. Crystallographers now have an extremely powerful tool, namely protein engineering, for the generation of nat...
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Applications of structural databases in the protein crystallographic structure determination process are reviewed, using mostly examples from work carried out by the authors. Four application areas are discussed: model building, model refinement, model validation and model analysis.
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I ventured into the field of protein crystallography in the early seventies, a few years after my undergraduate degree in physics from the Spanish educational system. This was a system where trespassing the boundaries of traditional disciplines was not encouraged. I was interested in biophysics as a general area of scientific enquiry and I needed to find a venue for my scientific interest and c...
متن کاملTriiodide derivatization in protein crystallography.
Methods for producing protein derivatives using cryosoak techniques with triiodide solutions are described. The methods have been tested using six different proteins. SAD/SIRAS phasing was attempted for each protein using data measured with conventional Cu Kalpha X-ray equipment and synchrotron radiation. Refinement of all six derivative structures showed that iodine is able to bind as I(-) (as...
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ژورنال
عنوان ژورنال: Nihon Kessho Gakkaishi
سال: 1987
ISSN: 0369-4585,1884-5576
DOI: 10.5940/jcrsj.29.1